Fig. 8. Role of Sesn in 2-DG-induced ECM degradation in human culture NP cells. Cells were treated with or without 2-DG (10 mM, 20 mM, and 40 mM) and combined with overexpression or knockdown of Sesn2. SMMP- 3, MMP-13, aggrecan, and COL2 proteins were detected by western blot analysis. GAPDH expression was detected as an endogenous control; Data are presented as the mean ± SD of three independent experiments. Data are presented as the mean ± SD. *P<0.05 vs. Vector, or si Scr group.